Banca de DEFESA: JOÃO PEDRO FERNANDES LENZ

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : JOÃO PEDRO FERNANDES LENZ
DATE: 30/01/2024
TIME: 14:00
LOCAL: https://meet.google.com/vdr-knyj-kub / Híbrida
TITLE:

TAXONOMIC IDENTIFICATION OF EMs (EFFICIENT MICROORGANISMS) UTILIZING DNA METABARCODING.  

KEY WORDS:

DNA metabarcoding; EM-solo; Brazilan Atlantic Rainforest; Social-technology; agroecology

PAGES: 66
BIG AREA: Ciências Biológicas
AREA: Genética
SUMMARY:

The utilization of microorganisms for nutritional and pest management purposes in Brazilian agriculture is becoming a common practice. The so called Efficient Microorganisms (EM), produced according to the methodology described in the "Caderno dos Microorganismos Eficientes (EM): Instruções práticas sobre o uso ecológico e social dos EM" (“The Book of the Efficient Microorganisms: practical instructions for the ecological and social use of EM”), are the basis for the production of the bioinoculant EM-solo. This methodology has been used by agroecology practitioners as a microbiome engineering tool. Biosafety issues have been a cause of concern for Brazilian regulators regarding “on farm” production of bio inoculants. In this study we produced the bioinoculant EM-solo, utilizing microorganisms captured in Atlantic rain forest in different regeneration status located in the region known as Sul da Bahia in Brazil. DNA extraction and amplification of the prokaryotic and eukaryotic metabarcodes, 16S (region V3V4) and ITS1 respectively were performed utilizing the primer pairs 341F/806R and ITSF/ITS2R. The amplicons were sequenced in the high throughput sequencing platform Illumina, generating 30 thousand paired reads (2x250 pb) per sample. The sequenced data were analyzed utilizing a pipeline combining USEARCH, CUTADAPT, DADA2 and Phyloseq packages for R. The results of this study demonstrate that EM-solo originated from all Atlantic Rain Forest regeneration status harbor microorganisms classified as plant growth promoters, but there were human pathogens present in all analyzed samples, indicating that farmers should use this product adopting certain biosafety measures.

COMMITTEE MEMBERS:
Externo à Instituição - ARISTOTELES GOES NETO - UFMG
Externa ao Programa - 1642871 - FLORISVALDA DA SILVA SANTOS - UFSBInterna - 1955705 - GABRIELA NAREZI
Presidente - 3074212 - THIAGO MAFRA BATISTA