Banca de QUALIFICAÇÃO: ANA PAULA OLIVEIRA SOUZA DIAS

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : ANA PAULA OLIVEIRA SOUZA DIAS
DATE: 08/06/2023
TIME: 14:00
LOCAL: CPF- meet.google.com/rqk-jstx-jgm
TITLE:

 Repellent activity of Piper macedoi Yunck essential oil against Aedes aegypti

KEY WORDS:

 Traditional knowledge; bioprospecting, cytotoxicity, genotoxicity, mutagenicity

PAGES: 2
BIG AREA: Ciências da Saúde
AREA: Medicina
SUMMARY:

Abstract: In Brazil, the increase in arboviral epidemics has been perpetuated as a serious public health problem. Diseases such as dengue, the reemergence of Yellow Fever, Chikungunya and Zika are associated with high rates of infestation by the Aedes aegypti mosquito. As a way of adding to the prevention of these diseases along with current vector control measures, encouraging the use of repellents by the population, especially based on natural products, can be of great value. The genus Piper with species native to the Atlantic Forest, such as Piper macedoi and Piper aduncum, showed promising results regarding insecticidal activity. Thus, the objective of the present study is to evaluate the repellent activity of the essential oil of P. macedoi against Aedes aegypti mosquitoes and to carry out a systematic review on Piperaceae species tested as repellents against A. aegypti. Essential oils were obtained from fresh leaves using the hydrodistillation method in a Clevenger-type apparatus, at the Interdisciplinary Laboratory II, UFSB -CPF. Virus-free Aedes aegypti eggs were donated by the Federal Institute's breeding center in Norte de Minas. The repellency test was approved by the Research Ethics Committee (>>>). The essential oil will be tested in 05 healthy volunteers, submitted to anamnesis and with absence of dermatological diseases and other significant comorbidities. The evaluation will be carried out after applying the formulations with the essential oil dilutions in the hand and forearm of the volunteers exposed to mosquitoes, created in a cage under control for the bioassays. Different concentrations of oils diluted in base cream will be tested: 0.25%; 1.25%; 2.50%; 3.75%, 5.0%; an industrial repellent positive control with DEET; and as a negative control, on the other hand/forearm, the application of non-ionized base cream. The evaluation will be carried out by counting the number of mosquito landings for 03 minutes without a break. The test will be performed in triplicate. The systematic review work showed that only a total of 12 articles with repellent assays against A. aegypti were carried out in Brazil, Thailand, Malaysia and India, stimulating further study. The essential oils tested were from eight species of Piperaceae: Piper aduncum (L), Piper betle (L), Piper longum (L), Piper nigrum (L), Piper marginatum (Jacq), Piper capitarianum (Yunck), Piper corcovadensis (Miq.) and Piper retrofractum (Vahl). The chemical profile showed a predominance of sesquiterpenes and phenylpropanoids among the major substances, however it indicates that a synergy between the compounds must be responsible for the repellent action.

COMMITTEE MEMBERS:
Presidente - 1822195 - DELIO JOSE MORA AMADOR JUNIOR
Interna - 1716815 - GISELE LOPES DE OLIVEIRA
Externa ao Programa - 3027379 - JANNAINA VELASQUES DA COSTA PINTO - nullInterna - 1197643 - MARCIA NUNES BANDEIRA RONER