Banca de QUALIFICAÇÃO: MARINA ROSA DE SOUZA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : MARINA ROSA DE SOUZA
DATE: 28/02/2023
TIME: 09:00
LOCAL: Sala 17 CPF / https://conferenciaweb.rnp.br/webconf/taina-soraiamuller
TITLE:

IN VITRO INDUCTION OF VANILLA PROTOCORMS (Vanilla Mill.) (ORCHIDACEAE)

KEY WORDS:

in vitro culture, Vanilla spp., orchid.

PAGES: 50
BIG AREA: Outra
AREA: Multidisciplinar
SUMMARY:

The genus Vanilla Mill., commonly known as baunilha, is one of the best known of the Orchidaceae family, due to its production of vanillin, an aromatic organic compound. The extracted oil has high commercial value, used in the food, pharmaceutical and cosmetics industries. Due to the intense activity of vanilla extraction for production, it is important to establish new methodologies for the application of conservation methods for the species. The use of biotechnology and tissue culture results in obtaining plants with higher quality and high multiplication rates, which can be obtained through organogenesis and somatic embryogenesis. Thus, the objective of the present work was to establish conditions for the in vitro cultivation of Vanilla spp., aiming at its propagation and conservation by inducing the formation of protocorms. The expedition in search of the species will be by the walking method, being demarcated and registered. For the in vitro germination experiment, different formulations of the culture medium Murashige and Skoog (MS) (1962) will be tested, being supplemented with 400 mg L–1 of glutamine and 80 mg L–1 of adenine sulfate, and 20 g L– 1 of D-Sucrose. In the propagation induction phase, the explants will be subjected to treatments for in vitro induction of protocorms via somatic organogenesis or embryogenesis, consisting of different concentrations of auxin-like growth regulators (2,4-D) in combination with different concentrations of auxin-like growth regulators. cytokinin-like growth (BAP). The propagules obtained from the induction will be inoculated containing 10 ml of MS culture medium, 30 g.L-1 of sucrose, 100 mg.L-1 of myo-inositol and solidified with 8 g.L-1 of agar. Two types of auxin (ANA and AIB) will be tested at different concentrations. The experimental design will be in randomized blocks. The analysis and interpretation of the data will be done in the SISVAR software. The data obtained will be submitted to analysis of variance and to the mean separation test (SNK 5%). It is expected that the results obtained from this work generate viable protocols for the in vitro propagation of Vanilla spp. protocorms, generating relevant information that allows the production of scientific articles, being disseminated in academic and popular circles.

COMMITTEE MEMBERS:
Externa à Instituição - ALANA ARAÚJO DOS SANTOS
Interno - 1216239 - ANDERS JENSEN SCHMIDT
Interno - 2250633 - FREDERICO MONTEIRO NEVES
Interna - 1156996 - LIVIA SANTOS LIMA LEMOS
Presidente - 1149467 - TAINA SORAIA MULLER