Evaluation of the antioxidant and photoprotective potential of three plant species collected in the Atlantic Forest of Southern Bahia
Plant extract, Photoprotection, UV radiation
Ultraviolet radiation has different wavelengths and can have different actions on organisms. Among the actions caused by this exposure are skin cancer, melasma and premature aging. To mitigate the harm caused by the incidence of solar radiation, there are substances with a photoprotective action, capable of reducing these impacts. Thus, several studies have evaluated the use of plants, with possible photoprotective activity. This incidence of sunlight, causes the production of oxidative compounds harmful to tissues, triggering problems of inflammatory disorder, which can lead to and / or aggravate various diseases. The plants, present diverse bioactive photoprotective compounds, with capacity to reduce the harmful impacts caused by the radiation, among them the phenolic ones. To evaluate this activity, phenolic compounds were extracted from three plant species from the Atlantic Forest in southern Bahia: Copaifera lucens Dwyer, Schnella angulosa (Vogel) Wunderlin,, Miconia albicans (S.W) Triana. Using centroid simplex planning, it aimed to identify the best solvent mixture (methanol, ethyl acetate and chloroform) in the extraction of phenolic compounds and to evaluate the photoprotective activity in vitro by ultraviolet spectrophotometry. The tests were performed in triplicate. Through the extraction carried out from the planning, it can be seen that in the Copaífera lucens Duyer, the ternary mixture of methanol, ethyl acetate and chloroform showed greater extraction of phenolics and in Miconia albicans (SW) Triana the binary mixture of methanol and acetate ethyl, was the one that best extracted phenolic compounds.